Hallworth and Michael G . Nichols Prestin in HEK cells is an obligate tetramer
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Prestin in HEK Cells is an 6 Obligate Tetramer 7 8 9
8 9 Richard Hallworth and Michael G. Nichols 10 Department of Biomedical Sciences, Creighton University School of Medicine, 11 Omaha, Nebraska 68178, USA 12 Department of Physics, Creighton University, Omaha, Nebraska 68178, USA 13 14 15 16 17 18 19 20 21 Running Head: Prestin is a Tetramer 22 Corresponding Author: 23 Richard Hallworth 24 Department of Biomedical Sciences 25 Creighton Universit...
متن کاملPrestin in HEK cells is an obligate tetramer.
The unusual membrane motor protein prestin is essential for mammalian hearing and for the survival of cochlear outer hair cells. While prestin has been demonstrated to be a homooligomer, by Western blot and FRET analyses, the stoichiometry of self association is unclear. Prestin, coupled to the enhanced green fluorescent protein, was synthesized and membrane targeted in human embryonic kidney c...
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Prestin (SLC26a5) is the outer hair cell integral membrane motor protein that drives cochlear amplification, and has been described as an obligate tetramer. We studied in real time the delivery of YFP-prestin to the plasma membrane of cells from a tetracycline-inducible cell line. Following the release of temperature block to reinstate trans Golgi network delivery of the integral membrane prote...
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The remarkable hearing sensitivity and frequency selectivity in mammals is attributed to cochlear amplifier in the outer hair cells (OHCs). Prestin, a membrane protein in the lateral wall of OHC plasma membrane, is required for OHC electromotility and cochlear amplifier. In addition, GLUT5, a fructose transporter, is reported to be abundant in the plasma membrane of the OHC lateral wall and has...
متن کاملThe roles of conserved and nonconserved cysteinyl residues in the oligomerization and function of mammalian prestin.
The creation of several prestin knockout and knockin mouse lines has demonstrated the importance of the intrinsic outer hair cell membrane protein prestin to mammalian hearing. However, the structure of prestin remains largely unknown, with even its major features in dispute. Several studies have suggested that prestin forms homo-oligomers that may be stabilized by disulfide bonds. Our phylogen...
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